New methods for Data Analysis of Isothermal Titration Calorimetry for studying binding of two n-alkyl Xanthates to Mushroom Tyrosinase

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New methods for Data Analysis of Isothermal Titration Calorimetry for studying binding of two n-alkyl Xanthates to Mushroom Tyrosinase

Author Affiliations

¹Department of Chemistry, Imam Khomeini International University, Qazvin, IRAN
² Department of Chemistry, Faculty of science, Islamic Azad University, Takestan branch, Takestan, IRAN
³ Institute of Biochemistry and Biophysics, University of Tehran, Tehran, IRAN

Res.J.chem.sci., Volume 2, Issue (4), Pages 76-78, April,18 (2012)

Abstract

A simple rapid direct isothermal titration calorimetry (ITC) method was applied to study the binding properties and structural changes of mushroom tyrosinase enzyme (MT) due to the interaction with two iso-alkyl dithiocarbonates (xanthates), C₃H₇OCS₂Na (I) and C₄H₉OCS₂Na (II) at 27 °C in phosphate buffer (10 mM) at pH 6.8. The extended solvation model provides more insights into this interaction for further understanding of the effect of iso-propyl and iso-butyl xanthate on the stability and the structural changes of MT. The solvation parameters derived from the solvation model can be related to the changes in the stability of enzyme and type of inhibition. ITC implies that there is a set of two binding sites for two new synthesized xanthates on MT with non cooperativity in the binding process.

References

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Seo S., Sharma V.K., and Sharma N., Mushroom Tyrosinase: Recent Prospects, J. Agric. Food Chem., 51, 2837-2853 (2003)
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Khyade Vitthalrao B. and Kulkarni Jyoti A., Effect of digoxin treated mulberry leaves on Protein profiles in fifth instar larvae of Silkworm, Bombyx mori (L) (PM x CSRâ‚‚), Res. J. Chem. Sci., 1(1), 2-7 (2011)
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Sonawane Vilas Y., Mechanistic study of chromium (VI) catalyzed oxidation of benzyl alcohol by polymer supported chromic acid, Res. J. Chem. Sci., 1(1), 25-30 (2011)

[ref1] Rescigno A., Sollai F., Pisu B., Rinaldi A., and Sanjust E. Tyrosinase Inhibition: General and Applied Aspects, J. Enzym Inhib. Med. Chem., 17, 207-218 (2002)

[ref2] Seo S., Sharma V.K., and Sharma N., Mushroom Tyrosinase: Recent Prospects, J. Agric. Food Chem., 51, 2837-2853 (2003)

[ref3] Amin E., Saboury A.A., Mansouri-Torshizi H., and Moosavi-Movahedi, Potent inhibitory effects of benzyl and p-xylidine-bis dithiocarbamate sodium salts on activities of mushroom tyrosinase, J. Enzym Inhib. Med. Chem., 25(2), 272-281 (2009)

[ref4] Saboury A.A., Enzyme Inhibition and Activation: A general theory, J. Iran. Chem. Soc., 6(2), 219-229 (2009)

[ref5] Alijanianzadeh M., Saboury A.A., Mansouri-Torshizi H., Haghbeen K., and Moosavi-Movahedi A A, The inhibitory effect of some new synthesized xanthates on mushroom tyrosinase, J. Enzym Inhib. Med. Chem., 22(2), 239-246 (2007)

[ref6] Rezaei Behbehani G., Divsalar A., Saboury AA. and Gheibi N. A new approach for Thermodynamic Study on binding some metal ions with human growth hormone, J. Solution Chem., 37(12), 1645-1655 (2008)

[ref7] Rezaei Behbehani G., and Mirzaie M., A high performance method for Thermodynamic Study on the Binding of Copper Ion anï¤ Glycine with Alzheiïer's aïylioï¤ Î² peptide, J. Therm. Anal. Cal., 96(2), 631-635 (2009)

[ref8] Rezaei Behbehani G., Divsalar A., Saboury A.A., Faridbod F. and Ganjali M.R., A new approach for Thermodynamic Study on the binding of Human Serum Albumin with Cerium Chloride, J. Bull. Korean Chem. Soc., 30(6), 1262-1266 (2009)

[ref9] Rezaei Behbehani G. and Barzegar L., Thermal study of lysozyïe binï¤ing with Î²-cyclodextrin, Applied Mechanics and Materials, 110, 1966-1969 (2012)

[ref10] Mirzaie M. and Rezaei Behbehani G., Thermal Study of the nickel ion Interaction with Myelin Basic Protein, Applied Mechanics and Materials, 110, 1963-19665 (2012)

[ref11] Khyade Vitthalrao B. and Kulkarni Jyoti A., Effect of digoxin treated mulberry leaves on Protein profiles in fifth instar larvae of Silkworm, Bombyx mori (L) (PM x CSRâ‚‚), Res. J. Chem. Sci., 1(1), 2-7 (2011)

[ref12] Vijayakumar R, Arokiaraj A. and Martin Deva Prasath P., Micronutrients and their Relationship with Soil Properties of Natural Disaster Proned Coastal Soils, Res. J. Chem. Sci., 1(1), 8-12 (2011)

[ref13] Yao Dongliang, Wang Yutian dong Yan, The Evaluation of Soil Cementation Generated from the Function of Microorganism, Res. J. Chem. Sci., 1(1), 13-17 (2011)

[ref14] Parmar Kokila, Prajapati Sarju, Patel Rinku Patel Rekha, A Simple and Efficient Procedure for Synthesis of Biologically Active 1,2,4-Triazolo-[3,4-b]-1,3,4-thiadiazole -2-aryl-thiazolidine-4-one Derivatives, Res. J. Chem. Sci., 1(1), 18-24 (2011)

[ref15] Sonawane Vilas Y., Mechanistic study of chromium (VI) catalyzed oxidation of benzyl alcohol by polymer supported chromic acid, Res. J. Chem. Sci., 1(1), 25-30 (2011)